Effects of Pre-Liminate™ & Biofilm Persistence on Surfaces

Validated Intervention by Pre-Liminate


Log10® studied the effect of Pre-Liminate™ probiotic powders on pathogenic biofilm persistence during the mechanical or abrasive action of dry dog food on both stainless steel and plastic surfaces. 


  1. Salmonella and Listeria biofilms were established in plastic and stainless steel cylindrical containers.  
  2. The cylinders were dusted with one of two probiotic formulas or with carriers without probiotics. 
  3. Dry dog food was introduced into the cylinders, and the cylinders were rotated at 10 rpm for 10 minutes.
  4. The dog food was recovered and tested for pathogens.
  5. The trial was repeated daily until the pathogen tests were negative.


  1. Pathogenic biofilm persistence on the plastic surface was greater in trials with Formula One (Figure 1) than with Formula Two (Figure 2), but this difference is not attributed to the probiotics.  Persistence on stainless steel was similar between the trials with Formulas One and Two.
  2. When no probiotic was applied, pathogenic biofilms remained for up to 10 days before the abrasion of the food on the biofilm finally removed it.
  3. When probiotic was applied, however, Salmonella and Listeria biofilms were removed two to six days faster.


The application of the dry probiotic blends was effective and consistent in removing an exceptionally large number (1000) of pathogen cells (both Salmonella and Listeria) on common food processing surfaces (stainless steel and plastic).  This biofilm containing a large number of pathogen cells would not likely ever be seen in practice.  With the smaller concentrations of pathogens found in typical plants, we believe that dry probiotics will be even more effective in eliminating and preventing pathogenic microorganisms in any food processing environment.

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